Asian Journal of Pharmaceutical Analysis

Saroj Kumar Raul ¹, Bukkuru Spandana ¹, Patibandla Sameera ¹, Vegiraju Vikitha ¹

Affiliations
1 Maharajah’s College of Pharmacy, Vizianagaram, Andhra Pradesh, IN

Abstract

A simple, sensitive, rapid, accurate, reproducible and less time consuming visible spectroscopic method has been developed for the determination of Gliclazide in pure and tablet dosage form. The wavelength maxima for gliclazide was found to be 224.05nm. Beer's law was obeyed in the concentration range of 2-20μg/ml. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.15μg/ml and 0.50μg/ml. The percentage recovery of the drug for the proposed method was 99.29% indicating no interference of tablet excipients. The results demonstrate that proposed method is accurate, precise and reproducible while being simple and rapid too for determination of gliclazide in tablet dosage form.

Keywords

Gliclazide, UV Spectroscopy, Method Development, Validation, Estimation.

Full Text

     

Saroj Kumar Raul ¹, Gopal Krishna Padhy ¹, Pramudula Ramya Krishna ¹, Boddu Uma Madhu Priya ¹

Affiliations
1 Maharajah’s College of Pharmacy, Vizianagaram, Andhra Pradesh, IN

Abstract

A simple, accurate, specific and precise UV Spectrophotometric method has been developed for estimation of valsartan in pure and pharmaceutical formulation. The λ_max of valsartan in methanol and water was found to be 248.21 nm. Methanol and water is used as diluent in equal proportion. The drug exhibited the linearity in the concentration range of 2-20μg/ml with correlation coefficient of 0.999. The % recovery of the drug for the proposed was found to be 99.2%. The limit of detection and limit of quantification was found to be 0.15% and 0.45% respectively. No interference was observed in the presence of common pharmaceutical excipient. The method was validated as per ICH guidelines. The developed method was successfully employed for the estimation of valsartan in pharmaceutical dosage form.

Keywords

Valsartan, UV Spectroscopy, Method Development, Validation, Estimation.

Full Text